The functional biochemistry of plants is very diverse. The concentrations of many compounds vary widely, and metabolomic analyses are required to determine all metabolites in plant extracts Among many analytical platforms, mass spectrometry is the most sensitive and selective technique, and thus it is the method of choice for metabolomic research on plants. LC-MS can be adapted to a wide range of molecules, such as secondary metabolites. Metabolomic studies coupled with chemometric methods including principal component analysis and partial least-squares regression analysis have been used to explore the relationships between the metabolome of diverse plant species and their genotype, origin, vintage, quality, or other specific attributes. Metabolic profiling techniques are often used to evaluate the nutraceutical value of a single plant cultivar for quality control and breading. In the field of nutraceutical research, such techniques have been used to identify subtle metabolic differences among individuals or among different environmental conditions, e.g., diet. However, to date, there has been little research on the use of metabolic profiling to Everolimus compare or predict the nutraceutical properties of many plant cultivars. Therefore, elucidating the relationship between the metabolome and the bioactivity of diverse cultivars could be a novel strategy for identifying the nutraceutical potential of various plant cultivars for functional food design. All the traditional cardiovascular risk factors are associated with endothelial dysfunction. Thrombin is a protease produced on the surface of injured endothelium from prothrombin circulating in the blood. It alters endothelial permeability by stimulating cell contraction through reorganization of the cytoskeleton. This increases the size of intercellular gaps and allows entry of inflammatory cells and atherogenic lipoproteins. A key event in the regulation of endothelial barrier function is actomyosin-driven contraction. Contraction of endothelial cells is initiated by Tubulin Acetylation Inducer Thr-18/Ser-19 phosphorylation of the 20-kDa myosin regulatory light chain , which is tightly associated with F-actin filament reorganization. Thrombin activity rapidly increases MRLC phosphorylation, stress fiber formation, and endothelial permeability. Thus, suppression of thrombininduced MRLC phosphorylation in ECs may improve endothelial dysfunction and may prevent progression of cardiovascular diseases such as atherosclerosis. Green tea has various health-promoting activities, and these activities vary from cultivar to cultivar. However, there is little information available for comparing numerous cultivars on the basis of their bioactivity.