There was interaction between Col and AuNPs. The decrease of the amide band intensity could be associated with the unwinding of the native collage triplehelical structure. For blood vessel tissue engineering, an ideal vascular scaffold should possess excellent biocompatibility and mechanical properties. AuNPs were demonstrated to have the ability to manipulate the microstructure of a synthetic or natural polymer even in very low concentration. The microstrucutural change of the polymer often led further to improved Fulvestrant biological functions. Besides, the size of nanoparticles may be critical to their effects. For example, larger silica nanoparticles did not interact strongly with collagen, whereas smaller ones formed rosaries along the protein fibers. Therefore, AuNPs were used in the current study and expected to interact with collagen. Vascular stents fabricated from the nanocomposites of collagen, poly by electrospinning had good mechanical properties, biocompatibility, and could guide tissue regeneration. Nanocomposites may provide a new way to build scaffolds for vascularized-tissue engineering. The morphogoical change of Col-Au 43.5 ppm increased the adhesion, proliferation, as well as migration of MSCs. Besides, Col-Au 43.5 ppm showed reduced inflammatory response and decreased platelet activation compared with the pure Col. These findings demonstrated that AuNPs had the potential to affect the biological performances of a matrix. Stem cell differentiation can be promoted by small molecules or appropriate ECM microenvironmental cues. In addition to providing a scaffolding support, ECM provides biological cues to the cell, activating cell signaling pathways that can trigger biological responses such as migration, proliferation, and survival. However, neither the patterns of stem cell differentiation triggered by different ECM components nor the mechanisms mediating this differentiation are well known. MSCs are a particularly intriguing cell source for tissue engineering because of their capacity of differentiation and secretion of bioactive factors that are both immunomodulatory and trophic interest in their therapeutic potential. In this study, we showed that Col-Au 43.5 ppm could modulate not only cellular morphological changes in MSCs, but also their functions. The mechanism by which nanotopography cues that affect stem cell behavior is thought to be related to the binding sites on ECMs and nanotopography-mediated cellular forces. We also observed that Col-Au 43.5 ppm could induce the the differentiation of MSCs into ECs.