Therefore, we studied time related characteristics of IR induced inflammation of the human small intestine in a newly developed human ischemia reperfusion model of the jejunum. The results indicate that reperfused ischemic jejunum effectively discards apoptotic and damaged epithelium, restores the epithelial gut barrier and thereby prevents the development of a local inflammatory response. The first part of surgery was according to standard procedures. From this moment the 6 cm part of jejunum, which was going to be studied, was identified and care was taken that the vasculature of the studied jejunum consisted of 1 central mesenteric arteriole and venule. This was achieved as described before. In brief, clamping and cutting of all collateral mesenterial vessels to the studied segment of jejunum, using Ultracision Harmonic Ace. Thereafter, the segment of jejunum was further Shionone isolated by transsection at both ends with a linear cutting stapler. The isolated jejunum was then subjected to 30 minutes ischemia using 2 a-traumatic vascular clamps, which are placed over the mesentery in opposed directions to ensure complete clamping. The isolated ischemic jejunum was subsequently kept in the abdominal cavity to guarantee warm ischemia. After half an hour of ischemia, one third of the isolated ischemic jejunum was resected to study early phenomena during ischemia. Reperfusion was initiated by removal of the clamps. Adequacy of reperfusion was confirmed by the gut regaining color and restoration of peristalsis. Subsequently, the LDE225 Diphosphate venous outflow was sampled. A further segment of isolated jejunum was resected similarly after 25 minutes of reperfusion to study early phenomena during reperfusion. The last part of studied jejunum was resected from 60 minutes after reperfusion onwards, to investigate late phenomena during reperfusion. At the time the last isolated reperfused segment of jejunum was obtained for the study, also 2 cm of jejunum, which had not been isolated and remained untreated during surgery, was resected using a linear cutting stapler.