The WST-1 assay is a colorimetric method based on the cleavage by mitochondrial dehydrogenase of the tetrazolium salt generating a detectable product, formazan. Two thousand cells for each MCF7 clone were seeded in 96-well plates in complete DMEM, with 2 wells devoid of cells as blank samples. Cells were allowed to seed overnight at 37uC in 5% CO2. Twenty-four hours after seeding, medium was changed with DMEM supplemented with 1% FBS. Two days after seeding, 10 ml of WST-1 reagent was added to the cells medium for 4 hours. The absorbance for the formazan product and the background control were recorded every hour by a multiplate reader . For each time point, the difference between the measures at 440 nm and 620 nm was calculated and inserted on a graph as a function of time. The slope of each curve was calculated and represented the cell proliferation index. For growth recording, 5.0 104 cells of each MCF7 clone were seeded in 6-well plates in complete DMEM. Medium was changed every 2 days. After 4, 7, 9, 11, 14 and 16 days of culture, cells were counted. Values were reported on a graph representing the cell growth of MCF7 clones. Non-alcoholic fatty liver disease is a spectrum of chronic diseases including fatty liver, or bland steatosis, as well as more aggressive lesions including steatohepatitis, lobular necroinflammation with fibrosis, or cirrhosis. NAFLD-related cirrhosis can lead to end-stage liver disease and hepatocellular carcinoma . Like other chronic end-stage liver diseases the major option for afflicted persons is liver transplantation. NAFLD appears to be associated with obesity and insulin resistance, and is considered the hepatic manifestation of the metabolic syndrome, along with type 2 diabetes 129-56-6 mellitus and certain dyslipidemias . An important component of Western diets includes both saturated fatty acids as well as trans-saturated fatty acids.