Our new automatic analysis using IIS made it possible to verify, as described before that this kinase is a hub involved in several biological processes through its interaction with diverse types of proteins in different cellular compartments, possibly at different time points during the cell cycle. In our previous work, we manually curate from the literature the hNek6 putative cellular roles,Cryptochlorogenic-acid considering all the novel interacting partners retrieved by the yeast two-hybrid screening, which were as follows: cell cycle, cytoskeleton organization, DNA repair, NFkappaB and Notch signalings and cancer-related interactions. Using our new approach, by building a network for the hNek6 interactions confirmed by in vitro and in vivo assays, DNA-dependent,Neochlorogenic-acid cell proliferation. A hallmark of epithelial cell-cell junctions is the tight junction. This structure forms a network of anastomosing intramembranous strands encircling the apico-lateral domain of the epithelial cell, eliminating the paracellular space between adjacent cells. This tight lateral seal is thus responsible for the epithelial paracellular permeability function. The epithelial tissue is one of the main types of tissue in the human body. It lines the external body and organ surfaces, providing a permeability barrier that protects against the external environment.